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Results about acquatic toxicity tests

Zebrafish embryo test to assess inertization of municipal solid waste incinerator residues by COSMOS technology

This work was realized in collaboration with the group of professor Marco Presta (University of Brescia)

One of the aim of the COSMOS-RICE project is to demonstrate that the obtained imert is safe.  To this purpose, a detailed protocol, based on the zebrafish embryo FET test has been developed to assess the toxicity of lixiviated MSWI fly ash and the corresponding inertized filler in zebrafish embryos. The results confirm that COSMOS technology provides a safe material, supporting the possibility that the inert may be safely used as second-hand material.

The following Figures summarize the obtained results.

 

 

Fig. 1. Effect of lixiviated MSWI fly ash or inertized COSMOS solution on zebrafish embryos.Representative embryos left untreated (controls) or exposed to the indicated concentrations of lixiviated MSWI fly ash or inertized COSMOS solutions were photographed at 24 hpf (a), 48 hpf (b) and 72 hpf (c). At all the time points investigated, MSWI fly ash-treated embryos show severe defects, including pericardial edema (white arrowhead), bent, short or almost completely absent tail (black arrowhead), deformed yolk sac (asterisk), smaller anomalous eyes (white arrow), not correctly protruded mouth (black arrow).Note that control and COSMOS-treated embryos underwent natural hatching at 72 hpf whereas chorion was manually removed from MSWI fly ash-treated embryos. No significant defects were observed in control and COSMOS-treated embryos at all the time points investigated.

 

Fig. 2. COSMOS inertization abrogates the toxic effects of lixiviated MSWI fly ash solution on zebrafish embryos. Embryos were exposed to the indicated concentrations of lixiviated MSWI fly ash or inertized COSMOS solutions. At the end of the FET test the mortality rate was calculated on 30 embryos per experimental point (a). Total General Morphology Score - GMS (b) and total General Teratogenic Score - GTS (c).

 
Zebrafish embryo is a complex, multicellular system characterized by the interaction of various tissues during developmental and differentiation processes. The proposed protocol, that includes the GMS/GTS assessment for different morphological/teratogenic endpoints, allows the accurate evaluation of the biotoxic effect of the substance(s) under test on various aspects of vertebrate development on different organs and apparata. 

In conclusion, in the present work the FET protocol, developped at the University of Brescia (prof. Marco Presta), is proposed for zebrafish embryos and successfully applied to demonstrate the chemical stabilization of heavy metal-containing waste using COSMOS technology. 

 See:

Biosafe inertization of municipal solid waste incinerator residues by COSMOS technology

Michela Guarientia, Alessandra Gianoncellib, Elza Bontempib, Sdenka Moscoso Cardozoa, Laura Borgeseb, Daniela Ziziolia, Stefania Mitolaa, Laura E. Deperob,∗∗, Marco Prestaa,

a Zebrafish Facility, Department of Molecular and Translational Medicine, University of Brescia, Viale Europa 11, 25123 Brescia, Italy b INSTM and Chemistry for Technologies Laboratory, University of Brescia, Via Branze 38, 25123 Brescia, Italy, 

Journal of Hazardous Materials 279 (2014) 311–321

 

 


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